mesenchymal stem cell growth medium dxf (PromoCell)

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PromoCell mesenchymal stem cell growth medium dxf
List of commercial serum-free media used

Mesenchymal Stem Cell Growth Medium Dxf, supplied by PromoCell, used in various techniques. Bioz Stars score: 94/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mesenchymal+stem+cell+growth+medium+dxf/Mesenchymal+Stem+Cell+Growth+Medium+XF/pmc10636218-6-2-9
Average 94 stars, based on 56 article reviews

mesenchymal stem cell growth medium dxf - by Bioz Stars, 2026-09

94/100 stars

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1) Product Images from "The Comparison of Commercial Serum-Free Media for Hanwoo Satellite Cell Proliferation and the Role of Fibroblast Growth Factor 2"

Article Title: The Comparison of Commercial Serum-Free Media for Hanwoo Satellite Cell Proliferation and the Role of Fibroblast Growth Factor 2

Journal: Food Science of Animal Resources

doi: 10.5851/kosfa.2023.e68

Figure Legend Snippet: List of commercial serum-free media used

Techniques Used:

(A) Cell proliferation on days 1, 3, and 6 of HWSCs cultured in six different serum-free media. Asterisks indicate significant differences between cells cultured in serum-free media and cells cultured in the control medium for each specific day. (B) Images of HWSCs cultured in SF and MS for 3 and 6 days. Scale bar=100 μm. (C) Total cell numbers and (D) PDLs of HWSCs cultured in SF and MS. Blue and orange asterisks indicate significant differences between SF and MS versus control medium at each time point, respectively. ** p<0.01, *** p<0.001. F10, Ham’s F-10 Nutrient Mix; Con, F10+20% FBS; NP, NutriStem hPSC XF Medium; CG, CellGenix® GMP Stem Cell Growth Medium; SF, StemFlex TM Medium; CT, Cellartis® MSC Xeno-Free Culture Medium 1 Kit; MS, Mesenchymal Stem Cell Growth Medium DXF; HWSC, Hanwoo satellite cell; FBS, fetal bovine serum.

Figure Legend Snippet: (A) Cell proliferation on days 1, 3, and 6 of HWSCs cultured in six different serum-free media. Asterisks indicate significant differences between cells cultured in serum-free media and cells cultured in the control medium for each specific day. (B) Images of HWSCs cultured in SF and MS for 3 and 6 days. Scale bar=100 μm. (C) Total cell numbers and (D) PDLs of HWSCs cultured in SF and MS. Blue and orange asterisks indicate significant differences between SF and MS versus control medium at each time point, respectively. ** p<0.01, *** p<0.001. F10, Ham’s F-10 Nutrient Mix; Con, F10+20% FBS; NP, NutriStem hPSC XF Medium; CG, CellGenix® GMP Stem Cell Growth Medium; SF, StemFlex TM Medium; CT, Cellartis® MSC Xeno-Free Culture Medium 1 Kit; MS, Mesenchymal Stem Cell Growth Medium DXF; HWSC, Hanwoo satellite cell; FBS, fetal bovine serum.

Techniques Used: Cell Culture, Control

(A) Protein expression of FGF2, p-ERK, and ERK in HWSCs cultured in SF and MS. (B) Changes in mRNA expression levels of biomarkers related to the FGFR1-mediated signaling pathway. (C) ICC staining of Pax7 and MyoD in HWSCs cultured in SF and MS. Pax7 is shown in green and MyoD in red. Nuclei are stained with Hoechst33342. Scale bar=100 μm. Significant differences compared with the control are indicated with an asterisks. * p<0.05, ** p<0.01, *** p<0.001. FGF2, fibroblast growth factor 2; p-ERK, phospho extracellular signal-regulated kinases; SF, StemFlex TM Medium; MS, Mesenchymal Stem Cell Growth Medium DXF; FGFR1, fibroblast growth factor receptor 1; FRS2, factor receptor substrate 2; Pax7, paired box 7; MyoD, myogenic differentiation; FGF2, fibroblast growth factor 2; HWSC, Hanwoo satellite cell; ICC, immunocytochemistry.

Figure Legend Snippet: (A) Protein expression of FGF2, p-ERK, and ERK in HWSCs cultured in SF and MS. (B) Changes in mRNA expression levels of biomarkers related to the FGFR1-mediated signaling pathway. (C) ICC staining of Pax7 and MyoD in HWSCs cultured in SF and MS. Pax7 is shown in green and MyoD in red. Nuclei are stained with Hoechst33342. Scale bar=100 μm. Significant differences compared with the control are indicated with an asterisks. * p<0.05, ** p<0.01, *** p<0.001. FGF2, fibroblast growth factor 2; p-ERK, phospho extracellular signal-regulated kinases; SF, StemFlex TM Medium; MS, Mesenchymal Stem Cell Growth Medium DXF; FGFR1, fibroblast growth factor receptor 1; FRS2, factor receptor substrate 2; Pax7, paired box 7; MyoD, myogenic differentiation; FGF2, fibroblast growth factor 2; HWSC, Hanwoo satellite cell; ICC, immunocytochemistry.

Techniques Used: Expressing, Cell Culture, Staining, Control, Immunocytochemistry

(A) Concentration-dependent HWSC proliferation promoted by FGF2. *** p<0.001 vs control. (B) Proliferative changes and representative images of HWSCs cultured for 3 days in MS and SF with 1 μM PD173074. Scale bar=100 μm. (C) Changes in mRNA expression levels of biomarkers associated with the FGFR1-mediated signaling in HWSCs induced by PD173074. (D) Changes in ERK and p-ERK protein expression in HWSCs induced by PD173074. * A significant difference from control; # A significant difference between groups with and without PD173074 treatment. (E) Schematic representation of the FGFR1-mediated signaling pathway in HWSCs. FGF2, fibroblast growth factor 2; SF, StemFlex TM Medium; MS, Mesenchymal Stem Cell Growth Medium DXF; FGFR1, fibroblast growth factor receptor 1; FRS2, factor receptor substrate 2; PBS, phosphate buffered saline; p-ERK, phospho extracellular signal-regulated kinases; HWSC, Hanwoo satellite cell.

Figure Legend Snippet: (A) Concentration-dependent HWSC proliferation promoted by FGF2. *** p<0.001 vs control. (B) Proliferative changes and representative images of HWSCs cultured for 3 days in MS and SF with 1 μM PD173074. Scale bar=100 μm. (C) Changes in mRNA expression levels of biomarkers associated with the FGFR1-mediated signaling in HWSCs induced by PD173074. (D) Changes in ERK and p-ERK protein expression in HWSCs induced by PD173074. * A significant difference from control; # A significant difference between groups with and without PD173074 treatment. (E) Schematic representation of the FGFR1-mediated signaling pathway in HWSCs. FGF2, fibroblast growth factor 2; SF, StemFlex TM Medium; MS, Mesenchymal Stem Cell Growth Medium DXF; FGFR1, fibroblast growth factor receptor 1; FRS2, factor receptor substrate 2; PBS, phosphate buffered saline; p-ERK, phospho extracellular signal-regulated kinases; HWSC, Hanwoo satellite cell.

Techniques Used: Concentration Assay, Control, Cell Culture, Expressing, Saline

(A) Images of HWSC differentiation (top) and ORO staining (bottom) after 4 days of differentiation. (B) Changes in mRNA expression levels of p38, MyoD, and MyoG on day 4 of differentiation. (C) Protein expression of Desmin and MyoG in differentiated HWSCs on day 4 of differentiation. (D) ICC staining of MyoD (red) and MyoG (yellow) in differentiated HWSCs on day 4 of differentiation. Nuclei are stained with Hoechst33342. Scale bar=100 μm. SF, StemFlex TM Medium; MS, Mesenchymal Stem Cell Growth Medium DXF; MyoD, myogenic differentiation; MyoG, myogenin; HWSC, Hanwoo satellite cell; ORO, Oil Red O; ICC, immunocytochemistry.

Figure Legend Snippet: (A) Images of HWSC differentiation (top) and ORO staining (bottom) after 4 days of differentiation. (B) Changes in mRNA expression levels of p38, MyoD, and MyoG on day 4 of differentiation. (C) Protein expression of Desmin and MyoG in differentiated HWSCs on day 4 of differentiation. (D) ICC staining of MyoD (red) and MyoG (yellow) in differentiated HWSCs on day 4 of differentiation. Nuclei are stained with Hoechst33342. Scale bar=100 μm. SF, StemFlex TM Medium; MS, Mesenchymal Stem Cell Growth Medium DXF; MyoD, myogenic differentiation; MyoG, myogenin; HWSC, Hanwoo satellite cell; ORO, Oil Red O; ICC, immunocytochemistry.

Techniques Used: Staining, Expressing, Immunocytochemistry

Cell Culture:

Article Title: The Challenge of Human Mesenchymal Stromal Cell Expansion: Current and Prospective Answers
Article Snippet: .. New Insights into Cell Culture Technology106 Product name Company Classification and comments (if information available by manufacturer) The literature and website CellGro® MSC Medium CellGenix SFM: albumin (human‐plasma‐derived) insulin (human recombinant, yeast‐derived), synthetic lipid contains chicken egg‐derived lecithin (licensed medicinal product for human use) Not cited Web: [136] CTSTM StemPro® MSC SFM Gibco by Thermo Scientific SFM: coating with CELLstartTM CTSTM necessary [77, 137, 138] Web: [139] hMSC High Performance Media Kit XF RoosterBio XFM: standardized, “enriched” basal medium Not cited Web: [140] Human MSC medium, chemically‐defined AmCell Biosciences recXFM: components are either chemically synthesized or recombinant produced and purified, none of its ingredients are directly derived from nonhuman animals Not cited Web: [141] Mesenchymal Stem Cell Growth Medium DXF (Defined Xeno Free) PromoCell XFM fibronectin‐coated plates are necessary [142] Web: [143] Mesenchymal Stem Cell Medium—animal component free (MSCM‐acf) ScienCell Research Laboratories XFM: no animal‐ or human‐origin materials. .. Contains (quantitatively and qualitatively formulated) growth factors, hormones and proteins Not cited Web: [144] MesenCultTM‐XF Medium STEMCELL Technologies SFM used in conjunction with the MesenCultTM‐SF Attachment Substrate Not cited Web: [145] MesenGro® Human MSC Medium System Biosciences/ StemRD recXFM: does not contain animal‐derived components, components are either chemically synthesized or recombinantly produced and purified Not cited Web: [146] MSC NutriStem® XF Medium Biological Industries XFM, defined formulation.

Article Title: Recombinant Spider Silk Functionalized with a Motif from Fibronectin Mediates Cell Adhesion and Growth on Polymeric Substrates by Entrapping Cells During Self-Assembly.
Article Snippet: Primary human smooth muscle cells (SMCs) isolated from coronary artery (ThermoFisher Scientific, Waltham, MA, USA) were cultured and Page 5 of 36 ACS Paragon Plus Environment ACS Applied Materials & Interfaces 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 6 expanded in complete smooth muscle cell growth medium (Gibco, Waltham, MA, USA) supplemented with 5% fetal bovine serum (FBS). .. Human mesenchymal stem cells (hMSCs) from bone marrow (ScienCell, Carlsbad, CA, USA) were cultured and expanded in ready-to-use mesenchymal stem cell growth medium DXF (PromoCell, Heidelberg, Germany) in flasks precoated with CELLstart (Gibco, Waltham, MA, USA). ..

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Synthesized:

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Purification:

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other:

Article Title: Serum‐free media for the production of human mesenchymal stromal cells: a review
Article Snippet: 4 , Mesenchymal stem cell growth medium DXF , C‐28019 , PromoCell , CD, SF, XF , Not cited.

Article Title: The Comparison of Commercial Serum-Free Media for Hanwoo Satellite Cell Proliferation and the Role of Fibroblast Growth Factor 2
Article Snippet: 7 , Mesenchymal Stem Cell Growth Medium DXF , PromoCell , MS.

Modification:

Article Title: Titanium phosphate glass microcarriers induce enhanced osteogenic cell proliferation and human mesenchymal stem cell protein expression
Article Snippet: .. Three types of culture media were used in different experiments, with all reagents procured from Gibco ® (Life Technologies Ltd, UK) unless mentioned otherwise: (1) low-glucose Dulbecco’s modified Eagle medium (DMEM) supplemented with fetal calf serum (FCS; 10% v v −1 ) and penicillin–streptomycin (1%); (2) osteogenic medium (OM), prepared as per previously explained methods and comprising low-glucose DMEM, FCS (10% v v −1 ), penicillin–streptomycin (1%), Fungizone (0.1%), dexamethasone (0.1 μM), ascorbic acid 2-phosphate (0.2 mM), and glycerol 2-phosphate (10 mM; last three chemicals procured from Sigma–Aldrich, UK); and (3) a commercially available mesenchymal stem cell growth medium DXF (PromoCell GmbH, Germany). .. Since DXF does not contain cell attachment and spreading factors, the surface of the cell culture flask was treated with bovine fibronectin (10 μg mL −1 ; PromoCell GmbH) in phosphate buffered saline (PBS) for 1 h prior to cell seeding in order to facilitate cell attachment and cytoplasmic spreading.

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